Design and Characterization of Maltose-Conjugated Polycaprolactone Nanofibrous Scaffolds for Uterine Tissue Engineering
نویسندگان
چکیده
Abstract Purpose Uterine anomalies are prevalent in women, and the major treatment assisted to them is hysterectomy as donor availability extremely low. To overcome this, engineering uterine myometrium smooth muscle tissue has become very important. Several studies have shown that polycaprolactone (PCL) nanofibers effective muscles, this type of scaffold structural similarities extracellular matrices cells. Here, we hypothesize by electrospinning PCL nanofibers, they form a suitable for engineering. Methods Polycaprolactone nanofibrous scaffolds were fabricated, surface modification was performed following two step wet chemistry method. First aminolysis which introduces primary amine groups on maltose conjugated scaffolds. This confirmed ninhydrin assay presence groups. followed ELLA where quantified. Modified further characterized scanning electron microscope (SEM), contact angle analysis Fourier transform infrared spectroscopy (FTIR). MTT assay, live-dead actin staining immobilization study improvement cell attachment proliferation rates modified Results Human fibroblast (HUF) cells displayed significant maltose-modified scaffolds, also exhibited appropriate morphology indicating these fibers highly growth. Conclusion Our results indicate fabricated (MPCL) would be potential biomaterial treat injuries promote regeneration. Lay Summary Future Work Over past few years, considerable efforts been directed towards design engineered could act human myometrial patch. We propose create fibroblast-based synthetic condition similar intrauterine microenvironment embryos embedded wall. For understanding efficiency patch, functional characterization will effects estrogen prostaglandins activity designed from experiments assist deeper how construct total bioengineered uterus can substitute transplantation procedure, nonetheless its initial stages development. Graphical
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ژورنال
عنوان ژورنال: Regenerative engineering and translational medicine
سال: 2021
ISSN: ['2364-4133', '2364-4141']
DOI: https://doi.org/10.1007/s40883-021-00231-0